The aim of the study was to evaluate the effect of Mat Pilates on the functional capacity (FC) of elderly women before and after six weeks of intervention. Eighteen women aged 62.28 (±2.34) participated in the study. Timed Up and Go test, Timed Up Stairs, Timed Down Stairs, 30-s Chair Stand, Chair Sit-and-Reach and Back Scratch tests were assessed. The results showed significant improvements in all FC tests after six weeks of the Mat Pilates intervention. Summarizing, only six weeks of Mat Pilates training of 60 min per session, three times a week, three series beginning with six repetitions and eight repetitions at the last two weeks of intervention, were enough to improve FC in elderlywomen. Furthermore, the exercises difficulty increased from beginners to intermediate.